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Enzyme-Linked Immunosorbent Assay (ELISA)

Topic updated on 05/24/17 4:56pm

Introduction
  • Purpose
    • to detect the presence of antigens via the use of antibodies that are conjugated with
      • an enyme that converts a colorless substrate (chromogen) into a colored end product
        • this is different than a radioimmunoassay (RIA) which
          • detects a radiolabeled product
  • Example
    • in an HIV screening test,
      • the HIV's p24 capsular antigen is coated onto a mirotiter plate
        • the patient's serum (which contains their antibodies) is added to this microtiter plate as well as
          • the anti-antibody that is labeled with the enzyme (added after washing)
            • the colorless substrate is added (after washing again), and if the reagents bind
              • a color change will occur

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Good animation describing the process
1/28/2016
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