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ELISA

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Topic updated on 03/21/15 7:23pm

Overview
  • ELISA = Enzyme-Linked ImmunoSorbent Assay 
  • Advantage
    • extremely sensitive test
      • detects presence of a protein or antigen
  • Disadvantage
    • high number of false positives
  • Mechanism
    • uses enzyme-linked color change to detect presence of protein/antigen
  • Clinical use
    • HIV screening test
  • Process of HIV screening test
    • HIV antigen coated on plate
    • test serum added
      • if antibodies are present they will bind to antigen
    • plate is washed
      • everything but bound antibodies will be washed away
    • anti-human gammaglobulin antibody added with bound color-producing enzyme
    • substrate for enzyme added
      • if HIV antibodies present in the serum there will be color present
  • Similar to Radioimmunoassay (RIA)
    • uses radiolabeling instead of color change

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Good animation describing the process
1/28/2016
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