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Fluorescence-Activated Cell Sorter (FACS)

Topic updated on 05/24/17 5:06pm

Introduction
  • Fluorescence-activated cell sorter (FACS)
    • separates a mixed population of cells after
      • they have been labeled with fluorescent dyes which subsequently
        • pass through a single-stream to then pass through a laser beam which
          • excites and thus fluoresces the dye
    • the scattered light created by the cell passing through this single-stream becomes detected by photomultiplier tubes
      • this provides information about
        • cell size and granularity
        • expression of cell-surface proteins on individual cells
    • within the cell sorter
      • cells can be separated based on their binding with fluorescently labeled antibodies


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